Antioxidant, Cytotoxic, Antidiabetic and Phytochemical Investigation of Root Extracts of Tecoma stans (Bignoniaceae)

Abstract

Introduction: The plants are an alternative source for the management of a number of human disorders due to the presence of biologically active constituents Aims: Current study aims to investigate underexplored therapeutic potentials of roots of Tecoma stans through phytochemical and biological assays. Methods: The dried roots were extracted successively with methanol and dichloromethane by simple maceration and labeled with codes TSM and TSD respectively. Results: The phytochemical investigation revealed the presence of alkaloids, flavonoids, saponins, terpenoids, tannins, andphenols in the extracts. TSM fraction revealed the highest total phenolic, flavonoid and alkaloid contents159.79 mg GAE/g, 65.38to 13.04 mg QE/g and95.2 to 56.61 mg AT/g of extract respectively. As compared to TSD extract, TSM extract showed significant antioxidant activity using two models DPPH (79.8 %) and ABTS (71.06 %). Cytotoxic activity of methanol extracts against PC3 and HeLa cancer cell line exhibited 61.23% (IC50 22.89 µg/mL) and 74.03 % (17.78 µg/mL) inhibition of cancer cells respectively at concentration of 30 µg/mL. Extracts revealed antidiabetic activity through α-amylase and α-glucosidase inhibition with maximum inhibition 63.26% and 75.66% respectively was detected at the concentration of 1000 mg / mL by TSM extract. Conclusion: Methanolic extracts of roots of Tecoma stanscontainshigher amount plant secondary metabolites and revealed antioxidant, cytotoxic and α-amylase as well as α- glucosidase inhibition activities.