On the dielectric method of monitoring cellular viability

Abstract

It is shown that the dielectric method is a valid way of monitoring cellular viability in situations where the mechanism causing death leads rapidly to gross cell lysis. We also show that the monitoring of capacitance at a single low radio frequency does reflect the actual dielectric properties of the cell suspension as judged from frequency scanning methods. It is tentatively suggested that the initial rise in capacitance of the suspension after the addition of the solvent is probably due to the cells’ size/membrane area increasing, rather than a direct effect of the solvent on the membrane permittivity. © 1993 IUPAC