The Effect of Medium Substitution on Cavendish Banana Shoot Multiplication (Musa acuminata L.)

Abstract

The Cavendish banana (Musa acuminata L.) is a highly significant cultivated plant for individuals living in tropical and subtropical regions. In vitro cultivation serves as a viable option for banana propagation, particularly for the Cavendish variety. The objective of this study is to identify the optimal medium for the in vitro multiplication of Cavendish banana shoots. This work used a method of research utilizing a single factor Completely Randomized Design, specifically involving a medium supplemented with cytokinin. The media were New Dogashima medium, Murashige and Skoog, Growmore Leaf Fertilizer, Sugihtani Liquid Organic Fertilizer, and DI Grow Liquid Organic Fertilizer. The treatment medium was supplemented with 3 mg/L of Benzyl Amino Purine, 0.1 mg/L of Naphthalene Acetic Acid, and 3 g/L of activated charcoal. Each treatment was replicated 3 times, with each replication comprising 3 samples, resulting in a total of 45 treatment units. The findings indicated that the Growmore leaf fertilizer medium, supplemented with 3 mg/L Benzyl Amino Purine and 0.1 mg/L Naphthalene Acetic Acid, together with 3 g/L activated charcoal, is the most effective medium for promoting banana shoot multiplication, thereby serving as a suitable alternative to synthetic medium.