Mitogenic response to TGF-β in 3T3-F442A cells

Abstract

In 3T3-F442A cells, TGF-β caused cellular proliferation in a time and dose-dependent manner. TGF-β induced cyclin D1 and cdk2 proteins in 3T3-F442A cells. The mitogenic effect of TGF-β was specific in nature. The antimitogenic agent, hGH, inhibited the mitogenic effect of TGF-β and was associated with inhibition of cyclin D1 expression. The protein kinase c inhibitor, staurosporine, inhibited the mitogenic effect of TGF-β. Taken together, these results suggest that TGF-β affects expression levels of cell cycle-regulated proteins and its mitogenic effect is mediated through protein kinase C in 3T3-F442A cells. © 1995 Academic Press, Inc.