Anti-Osteoporosis and Anti-Osteoarthritis Activity of Fresh Water Snail (Viviparous bengalensis) Flesh Extract in Experimental Animal Model

Abstract

Aims: Aim was to evaluate the anti-osteoporotic and anti-osteoarthritic activity of fresh water snail (Viviparous bengalensis) (VB) flesh extract (VBE) in experimental model. Settings and Design: Experimental osteoporosis (OSP) was developed in female Wistar rats by bilateral overectomy and Osteoarthritis (OA) was developed in male Wistar rats by bacterial collagenase injection. Methods and Material: VB was collected locally and authenticated, then homogenized in 3500 rpm × 15 mins and supernatant was collected. Rats were divided into-Group-1: Sham control, Group-2: OSP/OA control, Group-3: Standard (vitD3 200 mg·kg -1; p.o., calcium i.p. 1500 mg·kg -1 × 15 days in OSP and indomethacin 0.25 mg·kg-1, p.o. × 5 alternative days in OA), Group-4: VBE treated (1 gm·kg-1; p.o. × 15 days), Group-5: VBE treated (2 mg·kg-1; p.o. × 15 days). Anti-osteoporotic and anti-osteoarthritic activity of VBE was examined through physical, urinary and serum parameters. Statistical use: Data were expressed in terms of mean ± SEM (n = 6). ANOVA was performed, p Key findings: It was observed that the body weight, ankle/ knee diameters, urinary markers hydroxyproline/glucosamine/calcium/phosphate/creatinine, serum ACP/ALP/TRAP, calcium/creatinine, cytokines (TNF-α/IL-1β/CINC-1) levels were changed significantly and restored after VBE treatment. Significance: Fresh water snail flesh extract possess anti-osteoporosis and anti-osteoarthritic activity in experimental animal models.