A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein

Abstract

A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HGl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158°C for reaction times of 22.5 and 45 min with 7 M HC1 and 10% TFA containing 0.1 % phenol. © 1987, by the Japanese Biochemical Society.