Mouse Hematology: A Laboratory Manual

Abstract

Collection of peripheral blood -- Counting red blood cells, platelets, and viable nucleated white blood cells -- Protocol 1 determination of percent red cell volume (hematocrit) -- Protocol 2 methodology to determine a red blood cell count using a hemacytometer -- Protocol 3 methodology to determine a platelet cell count using a unopette and a hemacytometer -- Protocol 4 methodology to determine a white blood cell count and viability using a hemacytometer -- Peripheral blood films and cytospin preparations -- Protocol 5 washing slides in preparation for microscopic examination of peripheral blood and bone marrow -- Protocol 6 preparation of a peripheral blood film -- Protocol 7 fixing, staining, and mounting a peripheral blood film -- Protocol 8 preparation of a peripheral blood suspension for cytospin analysis -- Cell differential assessments of peripheral blood films -- Preparation of bone marrow for microscopic examination -- Protocol 9 bone marrow biopsy of the mouse -- Protocol 10 preparation of a bone marrow brush smear -- Protocol 11 preparation of a bone marrow suspension for cytospin analysis -- Cell differential assessments of bone marrow.