Genome mining of potential enzyme from Chelatococcus composti for sustainable production of D-Allulose

Abstract

A novel putative D-allulose 3-epimerase (DAEase) named CcDAE with the accession number of WP_183335203.1 was discovered in this study from Chelatococcus composti genome using in silico genome mining method. The sequence, which was retrieved from the genome of Chelatococcus composti strain CGMCC 1.15283 and became the first reported D-allulose 3-epimerase from the species. The sequence length of CcDAE was 282 aa with a molecular weight prediction of 30.504 kDa. The sequence analysis disclosed a high sequence conservation at the residues building the metal binding site and substrate binding site. The phylogenetic tree also disclosed that the closest related sequence with CcDAE was from Cereibacter spaeroides. The biochemical prediction also informed that CcDAE had an isoelectric point (pI) at pH 5.74. In addition, the novel putative enzyme was predicted to withstand a high temperature up to 65 °C and was considered as a stable protein. Therefore, the research finding suggests that CcDAE was potential for further exploration.