Comparative Evaluation of Salivary Enzyme in Patients With Gingivitis and Periodontitis: A Clinical-Biochemical Study

Abstract

Objective This study aims to relate aspartate aminotransferase (AST) in saliva and periodontal status in patients with gingivitis and periodontitis. Methods Forty-five patients have undergone a periodontal detailed examination as well as indexes sorted and classified into three gingival-based groups: healthy, gingivitis and periodontitis. Fifteen (15) patients were assigned for each group. Ten milliliter of stimulated saliva from a patient was collected after rinsing the mouth with 15 mL of water in a sterile tube. Biochemical analysis was conducted using the study GOT (ASAT) IFCC mod. liquiUV kit from HUMAN. Kinetic method for the determination of GOT (ASAT) activity and TC 84 Teco diagnostics chemistry analyzer. Result Acquired results indicated statically significant increases of AST level in saliva from patients with periodontitis and gingivitis (p < 0.01) in relation to the control group. Conclusion These results revealed that salivary AST level is higher in patients that have periodontal destruction, pocket depth and bleeding in probing. This clinically indicated that salivary biomarkers can be used as a diagnostic tool for the evaluation of periodontal health status.