Percentage Value of Membrane Integrity and Acrosome Integrity Spermatozoa in Simmental Liquid Semen with Addition Penicillin and Sweet Orange Essential Oil

Abstract

Growth of bacteria in the Simmental liquid semen is is the cause of the quality of liquid cement to be bad. The addition of antibiotics has been done to inhibit the growth of bacteria in the liquid semen. One of antibiotic that often used is penicillin. The addition of sweet orange essential oil is expected to further suppress the growth of bacteria because it contains antibacterial. The research objective was to determine the value of the percentage Membrane Integrity and Acrosome Integrity on Simmental liquid semen with the addition penicillin and sweet orange essential oil. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment was addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and penicillin extender. The results showed that addition sweet orange essential oil and tris yolk and penicillin extender resulted the best percentage value of the Membrane Integrity and Acrosome Integrity in Simmental liquid semen was the addition of sweet orange essential oil 1%.