Effect of mentha viridis L. Extracts on pathogenic bacteria adhesion

Abstract

Micro-organisms do not always exist in planctonic forms (single cells or small groups). To survive, especially in limiting media, they may adhere to inert or living surfaces. This enables them to multiply within a community protected by an extracellular matrix, thus forming a biofilm which protects them from antimicrobials agent especially antibiotics. In this paper, the aim of this study was to investigate in vitro the autoaggregation and coaggregation of resistant pathogenic bacteria responsible of intestinal infections (on young children), the surface hydrophobicity and the exopolysaccharides production treated with methanolic, hydro-methanolic and aqueous extract of Mentha viridis collected in South West of Algeria(wilaya of El Bayadh). The qualitative analysis revealed the presence of polyphenols and flavonoids, which were confirmed-by-quantitative-analysis. All extracts showed an antibacterial activity (anti-adhesiveness).The percentage of auto aggregation, co aggregation decreased in presence of extract comparing to control. E.coli was hydrophobic while the others hydrophilic. The amount of exopolysaccharides (EPS) produces by bacteria decreased considerably with all extracts comparatively to control (without extracts).