A novel deletion in KRT75L4 and KRT75 mediating the frizzle trait in an Indonesian frizzle chicken

Abstract

Indonesian frizzle chicken is one of the poultry species that have unique morphology. Morphological uniqueness in the form of curves from the ventral to the dorsal direction. In Java island, this chicken is believed to ward off negative power, rejecting magic. Frizzle Chicken is much more in demand among the others because of its uniqueness. This chicken also has a stronger body endurance. It is proven that frizzle chicken homozygotes have cells that can mediate the immune response in significant numbers to produce higher antibodies when the surrounding temperature is also high. It is related to the F gene expression that causes changes in the direction of hair growth increasing heat release. In Chinese Frizzle Chicken (Kirin), KRT75L4 gene mutation occurs. The purpose of this study was to explore whether Indonesian frizzle chicken has a similar mutation. Blood samples was used to isolate DNA from four Indonesian frizzle chicken originating from several regions in East Java. This treatment was made triplet, then PCR was traced and visualized through electrophoresis. It is predicted that Indonesian frizzle chicken is heterozygous and has a mutation in the KRT75L4 gene.