Media optimization in immobilized culture to enhance the content of Kaempferol in Tylophora indica (Asclepeadaceae) and Curcumin in Curcuma longa (Zingiberaceae).

Abstract

Immobilization of plant cell plays a very important role in exploiting plant cell culture for the production of high value phyto-pharmaceuticals. It is the advanced novel biotechnological approach, through which it is possible to achieve a physiological state conducive for metabolite production .That can overcome many limiting factors of suspension culture with distinct advantage of easier separation from product and also being agreeable for biotransformation of low value compounds to higher value product. In the described study, the immobilized culture of Tylophora indica (Asclepeadaceae) and Curcuma longa (Zingiberaceae) derived from leaves and rhizome respectively were used to enhance the production of their active compounds, Kaempferol and Curcumin. The maximum content of Kaempferol and Curcumin were obtained in cultures fed with 5mg/100ml of Cinnamic acid i.e.3.31%/300beads and 3.36 %/300beads respectively at the age of two weeks in Zenk media .This study also has given a clear image of biosynthetic pathway of these compounds.