Efficient heterologous production of atrochrysone carboxylic acid-related polyketides in an aspergillus oryzae host with enhanced malonyl-coenzyme a supply

Abstract

We recently developed an Aspergillus oryzae strain in which malonyl-coenzyme A (CoA) supply is strengthened by the deletion of snfA and SCAP as an efficient host to produce a plant polyketide, curcumin. Here, we examined the effectiveness of this strain in producing another polyketide, atrochrysone carboxylic acid (ACA), which is syn-thesized from eight molecules of malonyl-CoA us-ing an iterative type I polyketide synthase, ACA synthase (ACAS), and atrochrysone carboxyl ACP thioesterase (ACTE) in Aspergillus terreus. When ACAS and ACTE were introduced, the A. oryzae ∆snfA∆SCAP strain produced approximately four times more ACA-related polyketides than did the control strain expressing both genes. This result further demonstrated the availability of the A. oryzae ∆snfA∆SCAP strain for heterologous polyketide production.