High-Performace Liquid Chromatographic Determination of Mono- and Oligosaccharides in Vegetables with Evaporative Light-Scattering Detection and Refractive Index Detection

Abstract

Two high-performance liquid chromatographic methods are compared for the determination of mono- and oligosaccharides in vegetables using an NH2 column and acetonitrile-water as the mobile phase. One method uses a gradient elution and evaporative light-scattering detection; method precision (relative standard deviation) ranges from 1.09 to 4.44%, and detection limits range from 0.014 to 0.061 mg/mL. The second method uses isocratic elution and refractive index detection; method precision (relative standard deviation) ranges from 1.16 to 4.34%, and detection limits range from 0.013 to 0.022 mg/mL. When samples are analyzed by both methods, the difference between the two mean values is not statistically significant.