In-vitro cytotoxicity activity of Malaxis rheedii Sw methanol extract against hela cell line and MCF-7 cell line

Abstract

Objective: Cancer is a group of diseases caused by loss of cell cycle control. Cancer is associated with abnormal, uncontrolled cell growth. The study was aimed to evaluation of the anticancer activity of the Malaxis rheedii Sw. on the HeLa cell line and MCF-7 cell line. Methods: The whole plant parts of the M. rheedii methanolic extract were tested for its inhibitory effect on HeLa cell line and MCF-7 cell line. The cytotoxicity of M. rheedii on HeLa cell and MCF-7 cell line were evaluated by the 3-(4,5-dimethythiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Results: M. rheedii methanolic extract has a significant cytotoxicity effect on MCF-7 cell line in a concentration range between 18.75 and 300 µg/ml using MTT assay, and the study also showed that inhibitory action on HeLa cell line in a concentration range between 18.75 and 300 µg/ml using MTT assay. Methanol extract of the whole plant part of M. rheedii was found to be 7.3%, 16.6%, 25.4%, 36.3%, and 47.1% toxic in HeLa cell line and 7.9%, 13.9%, 26%, 48.4%, and 66.3% toxic in MCF-7 cell line. Inhibitory concentration 50 (IC50) value of M. rheedii on MCF-7 cell was 167.76 µg/ml, and IC50 value of M. rheedii on HeLa cell was not found by MTT assay. Conclusion: From the performed assay, the methanol extract of these drug shows greater activity on MCF-7 cell line and little activity on HeLa cell line and that mean M. rheedii can be used as an anticancer activity.