A critical comparison of techniques for estimating bacterial abundance on leaf litter decaying in Oued Zegzel (Morocco)

Abstract

A critical comparison of four techniques used to estimate the abundance and biomass of bacteria associated with leaves decaying in a small river in the North-East of Morocco is given. The relative efficiency of the four methods was as follows: homogenisation 〉 direct count 〉 stomacher 〉 sonication. However, each one of these techniques presents its advantages and disadvantages. No technique is perfect by itself.