A DNA polymerase with increased reactivity for ribonucleotides and C5-modified deoxyribonucleotides

Abstract

(Chemical Equation Presented) Just do it: Directed evolution enables efficient enzymatic synthesis of catalytically active RNA as well as highly functionalized C5-modified DNA. The DNA polymerase variant that is proficient in incorporating nucleobase and sugar-modified nucleotides was identified by a chip-based protein library screening assay. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.