Artificial Culture of an Entomogenous Nematode, Steinernema Kushidai (Nematoda: Steinernematidae)

Abstract

An entomogenous rematode, Steinernema kushidai, which is conspicuously lethal on white grubs, was cultured in artificial media. The nematode propagation was vigorous in the dog food agar medium supplemented with peptone, but poor in the non-supplemented medium. Analysis of the ammonia content of nematode cultured media indicated that the nematodes and/or symbiotic bacteria metabolized large amount of protein to propagate themselves. © 1989, JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY. All rights reserved.