Quantifying analysis of advanced glycosylation end products (ages) expression in periodontitis patients with diabetes type II

Abstract

Diabetes is an established risk factor for periodontitis. We investigated AGEs distribution in human gingival tissues and monocyte cytokine expression in diabetic patients with chronic periodontitis (DII/cP). Within indicated surgical procedures gingival biopsies were taken from 10 DII/cP and from 10 healthy controls. Tissue samples were prepared for immunohistochemistry using a monoclonal antibody specific for AGEs (6D12, TransGenic Inc.) and IMAGES J was used for quantification. Mean clinical parameters in DII/cP presented typical for severe chronic periodontitis (mt: 10.5; BoP: 84%; PBI: 2.9; cAL: 5.5 mm; rAL: 6.9 mm). In gingival tissue from DII/cP immunohistochemistry showed enhanced accumulation of AGEs (17%) compared to controls (13%). This study found that diabetes and periodontitis results in further AGE-accumulation compared to periodontitis alone. These results suggest the possibility that the blockade of RAGE may provide an effective approach in a range of diabetic complications including periodontal disease.