Bud development and shoot morphology in relation to crown location

Abstract

Plant architecture is shaped by endogenous growth processes interacting with the local environment. The current study investigated crown development in young black alder trees, assessing the effects of local light conditions and branch height on individual bud mass and contents. In addition, we examined the characteristics of parent shoots (stem cross-sectional area and total leaf area, shoot length, the number of nodes, the number and total mass of buds per shoot) and leaf-stem as well as bud-stem allometry, as several recent studies link bud development to hydraulic architecture. We sampled shoots from top branches and two lower-crown locations: one subjected to deep shade and the other resembling the upper branches in light availability. Sampling was carried out three times between mid-July and late October, spanning from the early stages of bud growth to dormancy. Individual bud mass and shoot characteristics varied in response to light conditions, whereas leaf-stem allometry depended on branch height, most likely compensating for the increasing length of hydraulic pathways. Despite the differences in individual bud mass, the number of preformed leaves varied little across the crown, indicating that the plasticity in shoot characteristics was mainly achieved by neoformation. The relationship between total bud mass and stem cross-sectional area scaled similarly across crown locations. However, scaling slopes gradually decreased throughout the sampling period, driven by bud rather than by stem growth. This suggests that the allometry of total bud mass and cross-sectional area of stem is regulated locally, instead of resulting from crown-level processes.