Multilocus sequence typing of Staphylococcus aureus with DNA array technology

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Abstract

A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.

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Van Leeuwen, W. B., Jay, C., Snijders, S., Durin, N., Lacroix, B., Verbrugh, H. A., … Van Belkum, A. (2003). Multilocus sequence typing of Staphylococcus aureus with DNA array technology. Journal of Clinical Microbiology, 41(7), 3323–3326. https://doi.org/10.1128/JCM.41.7.3323-3326.2003

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