Spectrophotometric Determination Of Anti-Ulcer Drug (Cimetidine) By 2.4-Dinitrophenylhydrazine Reagent

Abstract

An innovative, simple, and sensitive spectrophotometric method was described for the determination of cimetidine in both its pureform and in pharmaceutical formulations. By oxidizing 2.4-dinitrophenylhydrazine (2.4-DNPH) and mixing it with cimetidine in analkaline solution, a brightly colored chromogen with a maximum absorption wavelength of 586 nm is created. After that,spectrophotometry was used to quantify the amount of cimetidine. Other analytical criteria, such as the ideal reaction circumstances,were evaluated. These criteria included variables like the volumes of 2.4-DNPH and potassium iodate, the amount of base, thecoupling reaction time, temperature, and the order in which the constituents of the final product are added. Beer's law is applied toconcentrations of (25–250) µg/mL-1 with correlation coefficient (R2) = 0.9964, molar absorptivity 353.276 (L.mol-1.cm-1), andSandell's law. The recommended method for locating cimetidine in pharmaceutical formulations worked well.