Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik

Abstract

The development of seedless tomato fruits will be more attractive to both consumers and industries. Seedless tomatoes can be produced through parthenocarpy technology. Artificial parthenocarpy can be induced by conventional crossing, hormone application, or genetic engineering. The development of parthenocarpic tomatoes through genetic engineering has been carried out by inserting DefH9-iaaM parthenocarpic geneinto tomato genome via Agrobacterium tumefaciens mediated transformation. Sixty putative transgenic tomato lines were produced and three events (OvR1#14-4, OvM2#10-1, and OvM2#6-2) were selected as the best events. The background of the tomato lines was Oval variety, and based on PCR results, the three selected lines contained DefH9-RI-iaaM in their genome. The objective of this research was to determine the integration of DefH9-RI-iaaM gene in the progenies of three transgenic tomatoes lines using PCR technique. The research was conducted in the laboratory and Biosafety Containment Facility of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD). Parental variety, Oval (neither transgenic nor in vitro cultured), and elite line of CL 6046 were used as control plants. The results indicated that the progenies (T1, T2, and T3) of the three tomato lines contained the insert DefH9-RIiaaM gene.