Isolation and identification of flavonoid compounds from Curcuma’s rhizome (Curcuma aeruginosa Roxb.)

Abstract

This research was aimed to isolate and identify the flavonoid compounds from curcuma’ rhizome (Curcuma aeruginosa Roxb: Zingiberaceae). The extraction was carried out by Soxhlet method using petroleum eter, chloroform, nbutanol and methanol as the solvent agent. Those extract then qualitatively tested to identify the presence of flavonoid. Flavonoid then isolated from the extract of petroleum eter by column chromatography. The fraction resulted from chromatographic was analyzed by thin layer chromatography (TLC) method. Flavonoid identification was tested by color test, spectrophotometer UV-Vis, IR and GC-MS. The color test result showed that extract of petroleum eter, chloroform and n-butanol contain flavonoid. The analyzed single fraction from column chromatography showed that f2 contains isoflavone with 2 methoxy and 1 ethyl substitutes, f4 contains isoflavone with 2 methoxy substitutes, then f9 contains isoflavone with 1 hydroxy and 2 methoxy substitutes.