A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway

Abstract

Background:How the soluble receptor PEX5 delivers its cargoes to the peroxisome remains largely unknown. Results:Cargo translocation occurs after docking of the receptor at the peroxisome and before any ATP-dependent step. Conclusion:Translocation is concomitant with PEX5 insertion into the docking/translocation machinery. Significance:These results support a model in which cargoes are pushed across the peroxisomal membrane by PEX5. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.