Effect of ethanol extract and fractions of physalis calyx seu fructus on inflammation and mycoplasma gallisepticum

Abstract

The aim of the study was to investigate whether ethanol extract and fractions from D-101 macroporous resin of Physalis Calyx Seu Fructus has the anti-inflammatory and anti-Mycoplasma gallisepticum (MG) activity in vitro. Extract (P) was isolated from the dried calyces of Physalis Calyx Seu Fructus with 80% ethanol under reflux. The five major fractions (P1, P2, P3, P4 and P5) were obtained with ethanol gradient elution from D-101 macroporous resin column loaded with P. P and Fraction P2 was enriched with luteoloside content, with 1.39% and 2.548%, respectively. P3 had significantly inhibited the expression of IL-6, TNF-α and NO both at protein and gene level in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages by the qRT-PCR analysis and ELISA assay. Moreover, P2, P3, and P4 at its minimum inhibitory concentration inhibited the growth of MG. Conclusively, the present study suggested that P3 is a potential agent against MG and inflammation.