RNAzol® RT: a new single-step method for isolation of RNA

Abstract

The single-step RNA isolation with RNAzol® RT yields RNA ready for RT-PCR without additional purification or DNase treatment. Previous single-step methods required additional steps to remove DNA contamination before the isolated RNA was used in RT-PCR. RNAzol RT simplifies the single-step method and adds to its versatility and effectiveness. RNAzol RT isolates pure and undegraded RNA with protocols yielding either a single fraction containing total RNA or two separate fractions containing mRNA and small RNA.