Screening of agro residues for the production, purification and characterization of phytase enzyme from aspergillus ficuum mtcc 7591

Abstract

This work focus on the production of phytase from Aspergillus ficuum (MTCC 7591), which is one of the industrial important strain and also thermo stable in nature. Agro residues like rice bran (RB), wheat bran (WB), cotton seed oil meal (CSOM), coconut oil meal (COM), peanut oil meal (POM) and sesame oil meal (SOM) were used as a substrate for production of phytase through solid state fermentation (SFF). Among which coconut oil meal (22.4 U/gds & 38.45 U/ gds) shows maximum enzyme activity followed by wheat (17.86 U/gds & 32.56 U/gds) and sesame seed (19.6 U/gds & 25.36 U/gds). Phytase yield was increased with addition of salt solution to the substrate. The mixed substrate of coconut oil cake and wheat bran shows higher activity compare to individual titer value of 43.56 U/gds. The purification was done using ammonium sulphate precipitation and dialysis. The partial purified enzyme was subjected to SDS-PAGE analysis and it was found to be approximately 65kDa.