Trastuzumab-modified gold nanoparticles labeled with 211 At as a prospective tool for local treatment of HER2-positive breast cancer

Abstract

Highly localized radiotherapy with radionuclides is a commonly used treatment modality for patients with unresectable solid tumors. Herein, we propose a novel α-nanobrachytherapy approach for selective therapy of human epidermal growth factor receptor 2 (HER2)-positive breast cancer. This uses local intratumoral injection of 5-nm-diameter gold nanoparticles (AuNPs) labeled with an α-emitter ( 211 At), modified with polyethylene glycol (PEG) chains and attached to HER2-specific monoclonal antibody (trastuzumab). The size, shape, morphology, and zeta potential of the 5 nm synthesized AuNPs were characterized by TEM (Transmission Electron Microscopy) and DLS (Dynamic Light Scattering) techniques. The gold nanoparticle surface was modified by PEG and subsequently used for antibody immobilization. Utilizing the high affnity of gold for heavy halogens, the bioconjugate was labelled with 211 At obtained by α irradiation of the bismuth target. The labeling yield of 211 At was greater than 99%. 211 At bioconjugates were stable in human serum. Additionally, in vitro biological studies indicated that 211 At-AuNP-PEG-trastuzumab exhibited higher affnity and cytotoxicity towards the HER2-overexpressing human ovarian SKOV-3 cell line than unmodified nanoparticles. Confocal and dark field microscopy studies revealed that 211 At-AuNP-PEG-trastuzumab was eαectively internalized and deposited near the nucleus. These findings show promising potential for the 211 At-AuNP-PEG-trastuzumab radiobioconjugate as a perspective therapeutic agent in the treatment of unresectable solid cancers expressing HER2 receptors.