Sartobind STIC® salt-tolerant membrane chromatography

Abstract

Polishing in antibody production is usually performed on quaternary ammonium (Q) membranes in flow-through mode, since the overall speed and productivity is much higher than on traditional anion-exchange columns. However, the binding capacity of Q ligands is reduced at high conductivities, so concentrated feed streams must be diluted to adequately remove contaminants. Sartobind STIC PA (primary amine) anion-exchange membrane overcomes this limitation and can be used in high-salt conditions.