DIFFERENT ULTRAVIOLET SPECTROSCOPIC METHODS: A RETROSPECTIVE STUDY ON ITS APPLICATION FROM THE VIEWPOINT OF ANALYTICAL CHEMISTRY

Abstract

In routine practice, some simple and rapid analytical methods are needed for the assessment of formulations containing multiple elements, complex matrix system and for biotherapeutic products. There are several methods available for ultraviolet (UV) spectrophotometry that rely on the concept of absorbance difference, absorbance spectra, and additivity, also included in the list are simultaneous equation method, Q-absorbance ratio method, derivative spectrophotometry, ratio derivative spectra, successive ratio-derivative spectra, absorption and absorptivity factor method, and difference spectrophotometry along with multivariate chemometric methods. In this review, emphasis has been given to the theories, mathematical context, advantages, and disadvantages along with the vast applications of UV spectrophotometry. The findings further highlighted that for the analysis of drugs, UV spectrophotometry remains as one of the most simple, cheap, and promising option for routine practice in the field of pharmaceuticals.