Isolation and complete sequence of CBR, a gene encoding a putative cytochrome b reductase in Saccharomyces cerevisiae

Abstract

We have isolated and characterised a novel yeast gene, CBR (cytochrome b reductase), encoding a 35‐kDa yeast novobiocin‐binding protein. The predicted protein sequence of CBR displays considerable similarity to both plant nitrate reductases and mammalian cytochrome b5 reductases indicating that it is a putative member of the flavoprotein pyridine‐nucleotide‐cytochrome‐reductase family. Disruption of CBR is not lethal under various growth conditions, suggesting the presence of some functional overlap with other reductases, possibly with the cytochrome P‐450 reductase. Copyright © 1994, Wiley Blackwell. All rights reserved