Single-source biogenic AgNP–curdlan nanocomposite for endocrine disruptor dydrogesterone remediation

Abstract

This research reports the biosynthesis and application of a novel AgNP–curdlan nanocomposite using Priestia aryabhattai BMS3 for the effective removal of the endocrine-disrupting pharmaceutical pollutant, dydrogesterone, in water. The biosynthesis of silver nanoparticles (AgNPs) was validated through a distinct surface plasmon resonance peak at 432 nm, with a yield of 1.42 g/L. Concurrently, P. aryabhattai produced the exopolysaccharide curdlan at 96 h fermentation with a yield of 3.04 g/L. The nanocomposite was fabricated by integrating AgNPs into the curdlan matrix in a 1:5 ratio using ultrasonication and formed a stable reddish-brown film. The nanocomposite showed effective adsorption against dydrogesterone, attaining 92.45% and 91.13% removal as validated by UV-visible spectroscopy and HPLC, respectively. Experiments were carried out in synthetic wastewater at relatively high spiking levels. Results exhibit proof-of-concept, not the field evaluation.