Western Blotting (WB) Technique Guideline for Separation and Isolation of Protein

Abstract

A B S T R A C TWestern blotting is an important technique used in cell and molecularbiology. Using the western blot, researchers can identify specific proteinsfrom the complex mixture of proteins extracted from cells. This techniqueuses three elements to accomplish this task: (1) separation by size, (2)transfer to a solid support, and (3) marking target proteins using appropriateprimary and secondary antibodies to visualize. This paper will attempt toexplain the techniques and theory behind western blot, and offer severalways to solve the problem