Printed Capacitive Immunoassay for Detecting SARS-CoV-2 Viral Particles

Abstract

The key to fight against a global pandemic such as COVID-19 is to have low-cost, reliable, fast response diagnostic tools. Electronic biosensors are preferred because of their ease of integration into current centralized health care networks and integration with modern point-of-care testing (POCT) devices. Printed electronic sensors provide a sensitive and reliable diagnostic platform to aid in controlling transmissible diseases. In this work, we demonstrate a fully printed capacitive biosensor. The sensor uses coplanar electrodes, coupled with capture antibodies to detect the SARS-CoV-2 spike protein in spiked buffer solutions. Gold nanoparticle (GNP) tagged detection antibodies are also introduced to provide increased sensitivity. The gold nanoparticles provide a reflectance layer which leads to increased capacitance. This increased capacitance can be measured directly and has demonstrated the ability to screen for spiked samples with statistical significance. This fully printed capacitive immunoassay has the potential to be used as a transmissible disease screening and vaccine efficacy assessment tool for resource-limited areas.