Identification and suppression of secondary structures formed from deoxy-oligonudeotides during electrophoresis in denaturing polyacrylamide-gels

Abstract

Nature and stability of secondary structures formed from DNA-fragments during electrophoresis in urea containing polyacrylamide-gels were investigated. Duplices and especially hairpin loops were found to be surprisingly stable. Polyacrylamide-gels containing high concentrations of formamide effectively melt these structures. Such gels will improve nucleotide sequence analysis in regions of dyad symmetry that often cause 'band compressions' under standard electrophoresis conditions. © 1981 IRL Press Limited.