Identification of CD8+ T cell epitopes in the west nile virus polyprotein by reverse-immunology using netCTL

22Citations
Citations of this article
42Readers
Mendeley users who have this article in their library.

Abstract

Background: West Nile virus (WNV) is a growing threat to public health and a greater understanding of the immune response raised against WNV is important for the development of prophylactic and therapeutic strategies. Methodology/Principal Findings: In a reverse-immunology approach, we used bioinformatics methods to predict WNVspecific CD8+ T cell epitopes and selected a set of peptides that constitutes maximum coverage of 20 fully sequenced WNV strains. We then tested these putative epitopes for cellular reactivity in a cohort of WNV-infected patients. We identified 26 new CD8+ T cell epitopes, which we propose are restricted by 11 different HLA class I alleles. Aiming for optimal coverage of human populations, we suggest that 11 of these new WNV epitopes would be sufficient to cover from 48% to 93% of ethnic populations in various areas of the World. Conclusions/Significance: The 26 identified CD8+ T cell epitopes contribute to our knowledge of the immune response against WNV infection and greatly extend the list of known WNV CD8+ T cell epitopes. A polytope incorporating these and other epitopes could possibly serve as the basis for a WNV vaccine. © 2010 Larsen et al.

Cite

CITATION STYLE

APA

Larsen, M. V., Lelic, A., Parsons, R., Nielsen, M., Hoof, I., Lamberth, K., … Lund, O. (2010). Identification of CD8+ T cell epitopes in the west nile virus polyprotein by reverse-immunology using netCTL. PLoS ONE, 5(9), 1–11. https://doi.org/10.1371/journal.pone.0012697

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free