Improving the sensitivity of realtime PCR detection of Group B Streptococcus using consensus sequence-derived oligonucleotides

Abstract

Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from > 100 GBS genomes.