Effect of Acidic Challenge Preceded by Food Consumption on Enamel Erosion

Abstract

Objectives: This in vitro study aimed to evaluate the effect of food consumption followed by acidic challenge on enamel erosion.Methods: Seventy-five enamel blocks obtained from bovine teeth were divided randomly into five groups (n=15 per group): GI – erosion with previous immersion in milk; GII – erosion with previous immersion in cheese extract; GIII – erosion with previous immersion in liver extract; GIV – erosion with previous immersion in broccoli extract; and GV – erosive effect of cola drink (control). Over 24 h, the slabs were submitted to 3 pH-cycles, each consisting of immersion in the studied food (GI to GIV) for 5 min followed by immersion in a cola drink for 5 min, and subsequently, the slabs were stored in artificial saliva (110 min). At the end of the pH-cycles, the slabs were stored in artificial saliva for 18 h. Enamel alterations were assessed by profilometry (μm). Data were tested using ANOVA and Scott-Knott’s tests (P