Self-Assembly of Organelle-Localized Neuropeptides Triggers Intrinsic Apoptosis Against Breast Cancer

Abstract

Biosynthesis has become a diverse toolbox for the development of bioactive molecules and materials, particularly for enzyme-induced modification and assembly of peptides. However, intracellular spatiotemporal regulation of artificial biomolecular aggregates based on neuropeptide remains challenging. Here, an enzyme responsive precursor (Y1L-KGRR-FF-IR) is developed based on the neuropeptide Y Y1 receptor ligand, which self-assembles into nanoscale assemblies in the lysosomes and subsequently has an appreciable destructive effect on the mitochondria and cytoskeleton, resulting in breast cancer cell apoptosis. More importantly, in vivo studies reveal that Y1L-KGRR-FF-IR has a good therapeutic effect, reduces breast cancer tumor volume and generates excellent tracer efficacy in lung metastasis models. This study provides a novel strategy for stepwise targeting and precise regulation of tumor growth inhibition through functional neuropeptide Y-based artificial aggregates for intracellular spatiotemporal regulation.