Counter immunoelectro osmophoresis for the detection of infantile gastroenteritis virus (orbi group) antigen and antibody

Abstract

A moderately sensitive, rapid, and economical test scheme for the detection of infantile gastroenteritis virus (IGV) in stool or antibody in serum has been developed and evaluated. The test scheme with minor modifications was an adaptation of a counter immunoelectro osmophoresis system we once used for the detection of hepatitis B antigen. Large numbers of stool samples may be screened during half a working day for the pesence of IGV using reference antiserum to IGV prepared in guinea pigs. Serological studies of a diagnostic but not epidemiological nature may also be performed with equal facility by this same test scheme using highly purified IGV antigen derived from stool.