Quantification of Formaldehyde Residue in Wet Noodles Marketed in Indonesia using RP-HPLC Derivatization Method

Abstract

Illegal practices of formaldehyde as preservatives in wet noodles have been proven in West Java, Indonesia. Formaldehyde can cause a decrease of blood pressure, coma, acidosis, and acute renal failure. In this study we proposed a quantification method using high performance liquid chromatography (HPLC). A simulation was carried out as comparison. This simulation comprised of formaldehyde-spiked fish then the process was continued by washing, frying, and distilling the fish in a closed-system distillation. Similar condition was applied onto wet noodles samples. Method used was RP-HPLC derivatization method based on the reaction of formaldehyde carbonyl with two different reagents DNPH (2,4-dinitrophenylhydrazine) and Nash (acetyl acetone, ammonium acetate and acetic acid) reagents. Result showed that formaldehyde residue was detected and quantified in all wet noodle samples with a range of 21-59 ppm. In conclusion, this method can be used for routine analysis to control illegal practices of formaldehyde in wet noodles.