Genome-wide DNA polymorphisms in elite indica rice inbreds discovered by whole-genome sequencing

Abstract

Advances in next-generation sequencing technologies have aided discovery of millions of genome-wide DNA polymorphisms, single nucleotide polymorphisms (SNPs) and insertions-deletions (InDels), which are an invaluable resource for marker-assisted breeding. Whole-genome resequencing of six elite indica rice inbreds (three cytoplasmic male sterile and three restorer lines) resulted in the generation of 338million 75-bp paired-end reads, which provided 85.4% coverage of the Nipponbare genome. A total of 2819086 nonredundant DNA polymorphisms including 2495052 SNPs, 160478 insertions and 163556 deletions were discovered between the inbreds and Nipponbare, providing an average of 6.8 SNPs/kb across the genome. Distribution of SNPs and InDels in the chromosome was nonrandom with SNP-rich and SNP-poor regions being evident across the genome. A contiguous 4.3-Mb region on chromosome 5 with extremely low SNP density was identified. Overall, 83262 nonsynonymous SNPs spanning 16379 genes and 3620 nonsynonymous InDels in 2625 genes have been discovered which provide valuable insights into the basis underlying performance of the inbreds and the hybrids between these inbred combinations. SNPs and InDels discovered from this diverse set of indica rice inbreds not only enrich SNP resources for molecular breeding but also enable the study of genome-wide variations on hybrid performance. © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.