miR-339-5p inhibits cell migration and invasion in vitro and may be associated with the tumor-node-metastasis staging and lymph node metastasis of non-small cell lung cancer

Abstract

The aim of the present study was to investigate the potential role of microRNA (miRNA or miR) in invasion and metastasis of non-small cell lung cancer (NSCLC). miRNA-microarray analysis was used to detect the differentially expressed miRNAs between various metastatic levels of NSCLC cells. The microarray results were verified by quantitative polymerase chain reaction. The most clearly altered miRNA, miR-339-5p, was transfected into NSCLC cells and cell migration and invasion were investigated. The expression of miR-339-5p was 3.4662-fold higher in the lower metastatic NSCLC cells. miR-339-5p significantly decreased tumor-cell migration and the invasion capacity in vitro. In conclusion, miR-339-5p is important in NSCLC invasion and metastasis, indicating that miR-339-5p could be further evaluated as a biomarker for predicting the survival time of patients with NSCLC.