Gibberellin-Mediated Synergism of Xylogenesis in Lettuce Pith Cultures

  • Pearce D
  • Miller A
  • Roberts L
  • et al.
N/ACitations
Citations of this article
8Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Major gibberellins (GAs) in lettuce (Lactuca sativa L. cv Romaine) pith explants have been identified by gas chromatography-mass spectrometry (GC-MS) or GC-selected ion monitoring (GC-SIM) as GA(1), 3-epi-GA(1), GA(8), GA(19), and GA(20). Treatment of pith explants with indole-3-acetic acid (IAA) (57 micromolar) plus kinetic (0.5 micromolar) induced xylogenesis. In this xylogenic treatment, the concentration of a biologically active, polar GA-like substance(s) increased during the first 2 days of culture, although all of the above GAs decreased (as measured by GC-SIM). In non-xylogenic treatments, where explants were cultured without exogenous hormones, or with IAA or kinetin alone, the concentration of the biologically active, polar GA-like substance(s) decreased during the first two days of culture, as did all of the above GAs (as measured by GC-SIM). Treatment of pith explants with exogenous GA(1) alone did not induce xylogenesis, but GA(1) at very low concentrations (0.0014 and 0.003 micromolar) synergized xylogenesis in the IAA plus kinetin-treated cultures. These results suggest that changes in the concentration of certain endogenous GAs may be involved in xylogenesis mediated by IAA plus kinetin in lettuce pith cultures.

Cite

CITATION STYLE

APA

Pearce, D., Miller, A. R., Roberts, L. W., & Pharis, R. P. (1987). Gibberellin-Mediated Synergism of Xylogenesis in Lettuce Pith Cultures. Plant Physiology, 84(4), 1121–1125. https://doi.org/10.1104/pp.84.4.1121

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free