Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.)

Abstract

The biosynthesis path engineering could be very promising for mass production of alkaloids by applying elicitors in the cell suspension culture ofPersian poppy (Papaver bracteatum Lindl.). In this work, the effects of different concentrations of methyl jasmonate (MJ) and phloroglucinol (PG) on thebaineand sanguinarine productions in vitro were investigated. Roots as explant and supplementing 3 mg L-1 2,4-Dichlorophenoxyacetic acid with 0.5 mg L-1 Benzylamino purine to modifed MS medium were selected to achieve the most efcient combination for callus induction and production of callus fresh and dry weights.At 48 h after treatment, the addition of PG and MJ individually and in combination together signifcantly increased both thebaine and sanguinarine contents thanthe control. The results of high-performance liquid chromatography (HPLC) detection indicated that the highest production rate has been achieved through asynergic effect of two elicitors after 48 h. Results revealed that adding 200 μ M of MJ and 100 mg L-1 PG increased thebaine and sanguinarine contents by 56.36and 107.71-fold than control cells, respectively.