Design and chemical synthesis of a 2',4'-BNA probe for 7,8-dihydro-8-oxoguanine-containing double stranded DNA

Abstract

7,8-Dihydro-8-oxoguanine (8-OxoG), caused by DNA oxidation, is considered to be a marker of oxidative stress, and also to be a cause of genomic diversity in organism because of its mutagenic potential. Here, we designed and synthesized a 2',4'-BNA probe for 8-OxoG-containing double stranded DNA (dsDNA). The designed probe showed an 8-OxoG:C base pair selective triplex-forming ability.