Evaluation of immunochromatographic assay for serodiagnosis of bovine brucellosis in Gezira State, Sudan

Abstract

Aim: To assess the diagnostic value of the Immunochromatographic Assay (ICA) for serodiagnosis of bovine brucellosis and comparing results with those obtained from Rose Bengal Plate Test (RBPT) and Indirect Enzyme Linked Immunsorbent Assay (iELISA). Materials and Methods: In this study, 40 bovine sera were subjected to serological tests for bovine brucellosis using RBPT, iELISAand ICA using Anigen RapidS. Brucella Ab test kit. Results: Out of the 40 bovine sera examined, antibodies against Brucella were detected in 24 (60%), 27 (67.5%) and 20 (50%) samples by by RBPT, iELISA and ICA respectively. The kappa value between iELISA and ICA was 0.45 and that between RBPT and iELISA was 0.53. The sensitivity for RBPT and ICA was (76%) and (59.26%) respectively while specificity was (57.14%) and(80%) for these two tests respectively. Conclusion: These findings indicated that ICA was more specific than RBPT while the latter was more sensitive. Both tests showed almost moderate agreement. © The authors.