Isolation and characterization of batatasin III and 3,4'- dihydroxy-5-methoxybibenzyl: A pair of positional isomers from Sunipia scariosa

Abstract

Purpose: To isolate and characterize chemical compounds of biological importance from the whole plant of Sunipia scariosa. Methods: The whole plant of Sunipia scariosa was extracted with methanol (MeOH) and chromatographed on silica gel and sephadex LH-20 to afford the pure isolates. High perfomance liquild chromatography (HPLC) was used for further purification of the isolated compounds. Characterization of the isolated compounds was achieved by 1H and 13C nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). Results: Batatasin III (3,3'-dihydroxy-5-methoxybibenzyl) and 3,4'-dihydroxy-5-methoxybibenzyl, a pair of positional isomers, were isolated from the whole plant of Sunipia scariosa. The yields of the two isomers were 60 and 40%, respectively, from the mixture of two compounds. Conclusion: Batatasin III and 3,4'-dihydroxy-5-methoxybibenzyl, a pair of positional isomers were successfully isolated from the whole plant of Sunipia scariosa for the first time. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.